This page requires Adobe Flash Player to display correctly.
This ticker requires Adobe Flash Player to display correctly.

In Vivo Cell Culture Services

Custom Cell Preparation Stock Cell/Tissue Lysates

To permit our clients to fully benefit from our unique proteomics services without the hassles of cell culture, treatment and harvesting, subcellular fractionation, protein assay and shipping of frozen lysates, we are pleased to offer the convenience and cost savings of our In Vivo services. Clients can select from over two hundred pre-made cell and tissue lysates at no extra cost for use in our Kinex™ KAM antibody microarray or Kinetworks™ KCSS immunoblotting services. The cell lysates were prepared from our panel of 12 human tumour cell lines (described below) that have been incubated with diverse drugs and hormones in dose response and time course studies. The organs and tissues have been harvested from healthy monkeys, mice, rats and frogs.

We can also produce lysates from our human tumour cell lines prepared according to our clients’ requirements.
Send us your experimental compounds (e.g. drugs), proteins (e.g. cytokines and hormones) or oligonucleotides (e.g. RNAsi), and we will perform the treatments of the cells according to your specifications and generate lysates compatible for testing with any of our Kinex™ antibody microarray and Kinetworks™ immunoblotting services.

For use in our In Vivo services, we have selected 12 of the most commonly studied human tumour cells, which also provide broad representation for tissue source, gender and age of the originators. These cells were obtained at low passage number from the American Type Culture Collection. They have also received extensive previous characterization by Kinetworks™ analysis, and this data is available via the Internet from our KiNET databank.
The following is a listing of the current Kinexus human tumour cell line panel:

A431

Skin epidermoid carcinoma cells

A549

Lung carcinoma cells

HCT116

Colon carcinoma cells

HEK 293

Female fetal kidney cells

HeLa

Cervix epithelial adenocarcinoma cells

HepG2

Liver carcinoma cells – treated with insulin

HL-60

Peripheral blood promyeloblasts

HUV-EC

Umbilical vein endothelial cells

Jurkat

T cell leukemia cells

MCF-7

Breast epithelial adenocarcinoma cells

PC-3

Prostate adenocarcinoma cells

T98G

Brain glioblastoma cells
Credits