Stock Cell / Tissue Lysates
To permit our clients to fully benefit from our unique proteomics services without the hassles of cell culture, treatment and harvesting, subcellular fractionation, protein assay and shipping of frozen lysates, we are pleased to offer the convenience and cost savings of our In Vivo services. Clients can select from over two hundred pre-made cell and tissue lysates at no extra cost for use in our Kinex™ KAM antibody microarray or Kinetworks™ KCSS immunoblotting services. The cell lysates were prepared from our panel of 12 human tumour cell lines (described below) that have been incubated with diverse drugs and hormones in dose response and time course studies. The organs and tissues have been harvested from healthy monkeys, mice, rats and frogs.
We can also produce lysates from our human tumour cell lines prepared according to our clients’ requirements.
We can also produce lysates from our human tumour cell lines prepared according to our clients’ requirements.
Send us your experimental compounds (e.g. drugs), proteins (e.g. cytokines and hormones) or oligonucleotides (e.g. RNAsi), and we will perform the treatments of the cells according to your specifications and generate lysates compatible for testing with any of our Kinex™ antibody microarray and Kinetworks™ immunoblotting services.
For use in our In Vivo services, we have selected 12 of the most commonly studied human tumour cells, which also provide broad representation for tissue source, gender and age of the originators. These cells were obtained at low passage number from the American Type Culture Collection. They have also received extensive previous characterization by Kinetworks™ analysis, and this data is available via the Internet from our KiNET databank.
For use in our In Vivo services, we have selected 12 of the most commonly studied human tumour cells, which also provide broad representation for tissue source, gender and age of the originators. These cells were obtained at low passage number from the American Type Culture Collection. They have also received extensive previous characterization by Kinetworks™ analysis, and this data is available via the Internet from our KiNET databank.
The following is a listing of the current Kinexus human tumour cell line panel:
A431
Skin epidermoid carcinoma cells
A549
Lung carcinoma cells
HCT116
Colon carcinoma cells
HEK 293
Female fetal kidney cells
HeLa
Cervix epithelial adenocarcinoma cells
HepG2
Liver carcinoma cells – treated with insulin
HL-60
Peripheral blood promyeloblasts
HUV-EC
Umbilical vein endothelial cells
Jurkat
T cell leukemia cells
MCF-7
Breast epithelial adenocarcinoma cells
PC-3
Prostate adenocarcinoma cells
T98G
Brain glioblastoma cells
