The animations provided on this website are copyrighted by Kinexus Bioinformatics Corporation, but permission is given for their free use for personal and teaching purposes. Many of these animations were produced in collaboration with Cameron Bowyer (www.webmobilized.ca). They can be viewed using Quicktime Player. Any inquiries about these animations should be directed to Dr. Steven Pelech at Kinexus Bioinformatics Corporation (Phone: 604.323.2547 ext.10; e-mail: spelech@kinexus.ca).
Animations
Signal Transduction Proteins in Multimeric Complexes - Space Filling Models
These animations feature rotating 3D x-ray crystallographic structures of representative signalling proteins in complexes, which have been separately colourized to facilitate their visualization.
Download a full legend to protein chain colorization and detailed references for all the signal transduction proteins shown in animations immediately below
1A02
Fyn tyrosine kinase SH3 domain complexed with a synthetic peptide P2L corresponding to residues 91-104 of the p85 subunit of phosphatidylinositol 3- kinase
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1A37
14-3-3 bound to Raf kinase Ser-259 phosphorylation site containing peptide
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1AMP
cAMP-dependent protein kinase (PKA) catalytic subunit complexed with inhibitor portion of PKI inhibitor
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1AXC
Proliferating cell nuclear antigen (PCNA) binding to p21 WAF CDK inhibitor
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1BUH
Cyclin-dependent kinase 2 (CDK2) complex with cell cycle-regulatory protein Ckshs1
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1CVS
Fibroblast growth factor 2 (FGF2) dimer - fibroblast growth factor receptor1 (FGFR1) complex
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1E0A_2
Cdc42 complexed with the GTPase binding domain of p21-activated kinase (PAK)
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1E0O
Fibroblast growth factor 1 (FGF1) dimer - fibroblast growth factor receptor 2 (FGFR2) complex
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1ES7
Bone morphogenetic protein (BMP) receptor Ia extracellular domain bound to BMP-2
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1FAP
FKBP12-rapamycin complex interacting with PI3K-related kinase FRAP fragment
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1FPR
SHP-1 tyrosine phosphatase catalytic domain and an in vitro peptide substrate Py469 derived from SHPS-1
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1G1F
Protein tyrosine phosphatase 1B (PTP1B) complexed with a tri- phosphorylated peptide (Rdi(Ptr) Etd(Ptr)(Ptr)Rk) from the insulin receptor kinase
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1G6R
Superagonist T cell antigen receptor (TCR)/major histochemistry complex (MHC)
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1H10
Pleckstrin homology domain of Protein Kinase B/Akt bound to Ins(1,3,4,5)- tetrakisphosphate
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1H9O
Phosphatidylinositol 3-kinase (PI3K), p85- subunit: C-terminal SH2 domain complexed with a Tyr-751 phosphorylation site containing peptide from the platelet-derived growth factor receptor
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1JWH
Casein kinase 2 (CK2) holoenzyme alpha and beta subunit quaternary complex
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1KZ0
Protein farnesyltransferase complexed with farnesylated K- Ras4B peptide product and farnesyl diphosphate substrate bound simultaneously
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1N8Z
ErbB2 (HER2) receptor tyrosine kinase extracelleular domain complexed with herceptin monoclonal antibody Fab fragment
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1O6K
Protein kinase B/Akt kinase domain S474D complexed with glycogen synthase kinase 3 peptide and AMP-PnP
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1OWM
G protein coupled receptor kinase 2 (GRK2/BARK) complexed with G- beta/gamma subunits
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1Q4K
Polo-like kinase 1 (PLK1) polo-box domain in a complex with a phospho- peptide
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1RQQ
Insulin receptor tyrosine kinase in a complex with the SH2 domain of adapter APS anda substrate inhibitor
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1SHC
Shc PTB domain complexed with a phosphopeptide from the TrkA receptor- tyrosine kinase
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1WWW
Nerve growth factor in complex with domain 5 of the TrkA receptor-tyrosine kinase
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2NGR
Cdc42 bound to the active and catalytically compromised forms of the Cdc42-GTPase activating protein (GAP)
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CAM2
Calmodulin-dependent kinase 2 oligomer complexed with calmodulin (artistic composite - (CaMK2 catalytic domain represented with phosphorylase kinase catalytic domain)
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EGF
Epidermal growth factor (EGF) receptor- tyrosine kinase domain dimer shown with plasma membrane and extracellular EGF domain (artistic composite)
Click here to reviewSignal Transduction Proteins – Ribbon, Stick and Ball 3D Models
These animations feature rotating 3D x-ray crystallographic structures of cell signalling proteins with ribbon, stick and ball representation to facilitate their visualization.
Download a full legend to protein chain colorization and detailed references for all the signal transduction proteins shown in animations immediately below
1B39
Cyclin-dependent kinase 2 (CDK2) with Thr-160 phosphorylation site - ball model
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1E0A
Cdc42 complexed with the GTPase binding domain of p21-activated kinase-alpha (PAKa) - stick model
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1E8Z
Phosphatidylinositol 3-kinase gamma (PI3Kg) p110 catalytic subunit bound with inhibitor staurosporin - ribbon
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1EBA
Erythropoietin (Epo) receptor extracellular domain bound to Epo - ball model
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1F3M
p21-activated kinase-alpha (PAKa) autoregulatory domaincomplexed with its catalytic domain - ball and ribbon model
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1FMK
Src protein-tyrosine kinase with SH2, SH3, catalytic domain and C-terminus - wire model
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1FYN
Fyn protein-tyrosine kinase SH3 domain with proline-containing polypeptide from 3BP-2 - ball model
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1IR3
Insulin receptor-tyrosine kinase catalytic domain with peptide substrate - stick model
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1PTU
Protein-tyrosine phosphatase 1B complexed with a phosphotyrosine-containing hexapeptide - stick model
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1QJA
14-3-3 zeta dimer complexed with Raf1 phosphorylated serine-containing peptide - stick and ball model
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1QMZ
Cyclin-dependent kinase 2 (CDK2) complexed with cyclin A - ribbon and ball model
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4ERK
Extracellular-regulated kinase 2 (ERK2) complexed with drug - ribbon model
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Conserved Amino Acids in Catalytic Domains of Signal Transduction Proteins
These animations feature rotating 3D x-ray crystallographic structures of representative signalling proteins in complexes, which have been separately colourized to facilitate their visualization.
Download a full legend to protein chain colorization and detailed references for all the signal transduction proteins shown in animations immediately below
1QTN
Human Caspase 8 p18 (Residues 211-374) Human Caspase 8 p11 (Residues 385-473) (light grey) Substrate Peptide - IETD (dark grey)
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1UHO
Human cGMP-Specific 3’,5’-Cyclic Phosphodiesterase Catalytic Fragment Levitra (black) (Residues 537-860)
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1DJX
Rat Phosphoinositide-Specific Phospholipase C-delta1 complexed with Inositol-1,4,5-trisphosphate
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1AUI4
Human Protein-Serine Phosphatase 2B Calcineurin complexed with Calmodulin
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1G1G
Human Protein-Tyrosine Phosphatase 1B complexed with ETDYpYRKGGKGLL peptide from 1-866-kinexus TOLL FREE PHONE Insulin Receptor
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Signalling Protein Reaction Pathways
These animations feature rotating 3D x-ray crystallographic structures of representative signalling proteins in complexes, which have been separately colourized to facilitate their visualization.
AdrenRecGProtAdeCycl
Beta-adrenergic receptor activation of G proteins and stimulation of adenylate cyclase for production of cAMP
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ANFRecept.cGMPSignalling
Atrial naturitic factor induced dimerization of ANF receptor and activation of guanylate cyclase activity for production of cGMP
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CytokinesBathingCell
Distant view of cytokines bathing and activating a spherical cell
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PDGFRecept.Signalling
Platelet-derived growth factor induced dimerization and phosphorylation of PDGF receptor and activation of cell signalling
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ProteinPhosph_Reaction2
Protein phosphorylation reaction - A nano-satellite telemetry view - A kinase takes ATP and phosphorylates a protein substrate
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ProteinPhosph.Reaction1
Protein phosphorylation reaction - A kinase takes ATP and phosphorylates a protein substrate
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TGFbetaRecept.Signaling
Transforming growth factor-beta induced dimerization and phosphorylation of TGF-beta receptor and activation of cell signalling
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