PIMS
Protein Identification by Mass Spectrometry Services
Powerful and sensitive protein fingerprinting
Detect multiple phosphosites that are present in a purified phosphoprotein.
For more information on our protein identification by Mass Spectrometry Services please contact our technican services representatives toll free at 1-866-kinexus or by e-mail at info@kinexus.ca
Our Protein Identification by Mass Spectrometry (PIMS) Services are designed to permit our clients to establish the identities of cross-immunoreactive proteins that demonstrate interesting changes in expression or phosphorylation in their experimental model systems.
Such proteins are visualized with our Kinetworks™ multi-immunoblotting services and may also be detected with our Kinase-Substrate Profiling Services.
The PIMS service requires that the target cross-reactive protein can be immunoprecipitated or supplied in a purified form ready for trypsinolysis and resolution by LC-MS/MS mass spectrometry. These steps can be performed in-house at Kinexus for our clients.
Such proteins are visualized with our Kinetworks™ multi-immunoblotting services and may also be detected with our Kinase-Substrate Profiling Services.
The PIMS service requires that the target cross-reactive protein can be immunoprecipitated or supplied in a purified form ready for trypsinolysis and resolution by LC-MS/MS mass spectrometry. These steps can be performed in-house at Kinexus for our clients.
Since the
apparent molecular mass of the antibody cross-reactive
target protein is known, this can be used to further
validate the identification of the protein from the
estimated charge to mass ratios of its tryptic
fragments.
Such protein fingerprinting is a very powerful and sensitive strategy to identify proteins using our knowledge of the predicted charge to mass ratios of all of the proteins encoded by the genomes of humans and other species that have been sequenced.
Such protein fingerprinting is a very powerful and sensitive strategy to identify proteins using our knowledge of the predicted charge to mass ratios of all of the proteins encoded by the genomes of humans and other species that have been sequenced.